Journal: American Society of Clinical Oncology educational book. American Society of Clinical Oncology. Annual Meeting

Article Title: Liquid Biopsy to Identify Actionable Genomic Alterations

doi: 10.1200/EDBK_199765

Figure Lengend Snippet: Survival Outcome (Progression-Free Survival, Overall Survival, and Survival After Osimertinib Progression) of EGFR T790M Shedders Versus Nonshedders

Article Snippet: 30 The four additional tests reviewed, in addition to the three government-approved tests, were the AmoyDx EGFR 29 mutation detection kit, AmoyDx Super-ARMS EGFR T790M mutation detection kit, Droplet Digital PCR (ddPCR) Dx system (Bio-Rad, Hercules, CA), and PANA Mutyper R EGFR kit (Panagene, Daejeon, Korea).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers

doi: 10.3390/ijms23158386

Figure Lengend Snippet: WGA methods.

Article Snippet: MDA and improved MDA [ , ] , Random priming and isothermal exponential amplification, Phi29 or Bst polymerases , Qiagen REPLI-g Single Cell Kit, GE GenomiPhi DNA Amplification Kit, AmpliQ Genomic Amplifier Kit, Sygnis TruePrime WGA kit , Less uniformity, artifact of C>T transitional mutation, non-reproducible from cell to cell, low chimera rate , More genome coverage (80%), low FP and FN, compatible with digital droplet MDA , Mutation detection, SNP.

Techniques: Degenerate Oligonucleotide–primed Polymerase Chain Reaction, Amplification, Multiple Displacement Amplification, Mutagenesis, Multiple Annealing and Looping–Based Amplification Cycles

Journal: Biosensors

Article Title: Longitudinal Study of Circulating Biomarkers in Patients with Resectable Pancreatic Ductal Adenocarcinoma

doi: 10.3390/bios12040206

Figure Lengend Snippet: KRAS mutation in tumor tissue or in plasma detected using ddPCR.

Article Snippet: Droplet digital PCR (ddPCR) was carried out at Atila Biosystems (Mountain View, CA, USA). ddPCR and qPCR methods were developed and carried out independently, without knowing each other’s results before experiments. cfDNA was extracted from 2 mL of human plasma using a QIAamp MinElute ccfDNA Midi Kit (Qiagen; Hilden, Germany) according to the manufacturer’s instructions with an elution volume of 40 μL. ddPCR was carried out using the KRAS Multiplex Mutation Screening Kit (Atila Biosystems; Mountain View, CA, USA) according to the manufacturer’s instructions.

Techniques: Mutagenesis, Clinical Proteomics